Lactobacillus rhamnosus GG inhibits Cronobacter-induced meningitis in neonatal rats / 南方医科大学学报
Journal of Southern Medical University
; (12): 1079-1083, 2015.
Article
em Zh
| WPRIM
| ID: wpr-333678
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the inhibitory effect of Lactobacillus rhamnosus GG ( LGG) against Cronobacter-induced meningitis in neonatal rats.</p><p><b>METHODS</b>The cell adhesion and invasion capacities of Cronobacter were assayed in Caco-2 cells, and the optimal time length and concentration of the bacterium for infection were determined. The suppressive effects of LGG on the adhesion and invasion of Cronobacter in caco-2 cells were tested by competitive and exclusion experiments, and its inhibitory effect against Cronobacter-induced meningitis was evaluated in neonatal rats.</p><p><b>RESULTS</b>Cronobacter showed aggressive adhesion to caco-2 cells with an optimal infection time of 3 h. LGG produced a concentration-dependent inhibition of Cronobacter adhesion and invasion by competing with and excluding the latter for cell adhesion. In neonatal rats, LGG showed an obvious preventive effect and also a moderate therapeutic effect against Cronobacter-induced meningitis.</p><p><b>CONCLUSION</b>LGG can inhibit Cronobacter entry across the intestinal barrier to achieve preventive and therapeutic effects against Cronobacter-induced meningitis.</p>
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Índice:
WPRIM
Assunto principal:
Terapêutica
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Virulência
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Aderência Bacteriana
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Meningites Bacterianas
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Células CACO-2
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Probióticos
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Infecções por Enterobacteriaceae
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Lacticaseibacillus rhamnosus
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Cronobacter
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Intestinos
Limite:
Animals
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Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Ano de publicação:
2015
Tipo de documento:
Article