Your browser doesn't support javascript.
loading
Interleukin-2 reduces calcium handling capacity of rat ventricular myocytes during anoxia/reoxygenation / 中国应用生理学杂志
Chinese Journal of Applied Physiology ; (6): 111-115, 2004.
Artigo em Chinês | WPRIM | ID: wpr-333701
ABSTRACT
<p><b>AIM</b>To investigate the effect of interleukin-2(IL-2) on the cell contractility and calcium handling in cardiomyocytes during normoxia or anoxia/reoxygenation.</p><p><b>METHODS</b>Chemical anoxia introduced by Krebs-Henseleit(K-H) solution containing 10(-3) mol/L sodium dithionite was used in the enzymatically isolated rat ventricular myocytes. The video-tracking system and spectrofluorometric method were employed to verify the cell contraction and calcium handling of the single myocyte.</p><p><b>RESULTS</b>During anoxia, the cell contraction, amplitude of calcium transient induced by electrical stimulation and Ca2+ release induced by caffeine were depressed while resting calcium level was elevated, but the activity of the L-type calcium channels were not changed. All the parameters could not return to the pre-anoxia level during reoxygenation. IL-2 at 2 x 10(5) U/L administrated during anoxia aggravated the effect of reoxygenation on cell contraction and the calcium handling.</p><p><b>CONCLUSION</b>Coexistence of IL-2 during anoxia aggravated the effect of reoxygenation on the cell contraction and calcium handling in the isolated rat ventricular myocytes, in which the reduced calcium release from sarcoplasmic reticulum was involved.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Oxigênio / Farmacologia / Retículo Sarcoplasmático / Hipóxia Celular / Células Cultivadas / Cálcio / Interleucina-2 / Ratos Sprague-Dawley / Biologia Celular / Miócitos Cardíacos Limite: Animais Idioma: Chinês Revista: Chinese Journal of Applied Physiology Ano de publicação: 2004 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Oxigênio / Farmacologia / Retículo Sarcoplasmático / Hipóxia Celular / Células Cultivadas / Cálcio / Interleucina-2 / Ratos Sprague-Dawley / Biologia Celular / Miócitos Cardíacos Limite: Animais Idioma: Chinês Revista: Chinese Journal of Applied Physiology Ano de publicação: 2004 Tipo de documento: Artigo