Hypoxic preconditioning increase nPKCepsilon membrane translocation in the brain of mice / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 105-109, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-333703
ABSTRACT
<p><b>AIM</b>To explore the role of novel protein kinases C (nPKCs) in the development of cerebral hypoxic preconditioning.</p><p><b>METHODS</b>By using the mice model of hypoxic preconditioning, which was established before in our lab, the biochemistry techniques of SDS-PAGE and Western blot were applied to observe the effects of repetitive hypoxic exposure (H0-H4) on nPKCs (nPKCepsilon, delta, eta, mu and theta) membrane translocation in hippocampus and cortex.</p><p><b>RESULTS</b>nPKCepsilon membrane translocation was increased in response to the hypoxic exposure times in the hippocampus (H0 41.6% +/- 1.4% vs. H1-H4 46.9% +/- 4.5%, 52.7% +/- 3.9%, 58.8% +/- 2.7% and 61.3% +/- 3.7%) and cortex (H0 38.4% +/- 4.5% vs. 42.4% +/- 5.0%, 48.7% +/- 6.5%, 55.3% +/- 8.9% and 61.2% +/- 10.2%) of mice, and there were statistic significances among H2, H3 and H4 in hippocampus, and H3 and H4 in cortex respectively (P < 0.01). But for nPKCdelta, eta, mu and theta membrane translocation, there were no any significant changes in hippocampus and cortex of hypoxic preconditioned mice.</p><p><b>CONCLUSION</b>nPKCepsilon may play an important role in the development of cerebral hypoxic preconditioning, but it need more evidence to prove.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Encéfalo
/
Proteína Quinase C
/
Western Blotting
/
Transporte Proteico
/
Hipocampo
/
Hipóxia
/
Metabolismo
/
Camundongos Endogâmicos BALB C
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2004
Tipo de documento:
Artigo
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