Effects of hsf1 genotype on the constitutive expression of alphaB-crystallin in mice myocardium / 中国应用生理学杂志

ABSTRACT

<p><b>AIM</b>Try to clarify the effects of HSF1 gene on the constitutively expressed alphaBC.</p><p><b>METHODS</b>To investigate the levels of constitutively expressed alphaB-Crystallin (alphaBC) in hsf1 knockout (hsf1 -/-) and hsf1 wild type (hsf1 +/+) mice myocardium by Western blot and immunohistochemistry.</p><p><b>RESULTS</b>The alphaBC levels in hsf1 -/- and hsf1 +/+ were 68.42% +/- 4.16%, 100% +/- 7.58%, respectively (P < 0.05, cytosolic fraction), and 20.53% +/- 1.01%, 37.55% +/- 1.91%, respectively (P < 0.05, pellet fraction). The alphaBC signals decreased significantly in hsf1 -/- myocardium compared with hsf1 +/+ myocardium stained with fluorescence immunohistochemistry.</p><p><b>CONCLUSION</b>hsf1 is the important, but not the only factor, which mediates the constitutively expressed alphaBC.</p>