Effects of sodium on rat osteoblast and the role of epithelial sodium channel / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1871-1874, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-333793
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of sodium on rat osteoblast function and explore the role of epithelial sodium channel (ENaC) in such effects.</p><p><b>METHODS</b>The proliferation and differentiation of rat osteoblasts were evaluated following treatment with 1×10(-4) mol/L to 1 mol/L Na(+). The mRNA expressions of the osteogenic genes and ENaC-α gene in the treated cells were assessed using RT-PCR.</p><p><b>RESULTS</b>Within the concentration of 1×10(-4) mol/L to 1 mol/L, Na(+) showed a two-way effect on the osteoblasts low-concentration Na(+) (1×10(-4) mol/L) significantly promoted osteoblast differen- tiation, while at higher concentrations (0.5 and 1 mol/L), Na(+) produced an opposite effect. Sodium did not significantly affect osteoblast proliferation. Low-concentration Na(+) significantly increased the transcription of Cbfa1, OPN and OC, while high concentrations of Na(+) decreased their transcription. Low-concentration Na(+) also enhanced the mRNA expression of ENaC-α, but high-concentration Na(+) treatment lowered ENaC-α mRNA expression.</p><p><b>CONCLUSION</b>Na(+) displays a direct dose-related effect on osteoblasts by affecting its differentiation, osteogenic gene expression profile, and ENaC-α gene expression, suggesting the involvement of ENaC in Na(+)-mediated functional modulation of rat osteoblasts.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteoblastos
/
Farmacologia
/
Cloreto de Sódio
/
Diferenciação Celular
/
Células Cultivadas
/
Ratos Sprague-Dawley
/
Biologia Celular
/
Proliferação de Células
/
Canais Epiteliais de Sódio
/
Transcriptoma
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2011
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS