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Establishment of transgenic mice with visualized neovascularization / 南方医科大学学报
Journal of Southern Medical University ; (12): 1748-1752, 2011.
Artigo em Chinês | WPRIM | ID: wpr-333822
ABSTRACT
<p><b>OBJECTIVE</b>To establish transgenic mice with GFP expression in the vascular endothelium during neovascularization.</p><p><b>METHODS</b>The vector nestin-hsp68-gfp containing nestin second intron was introduced into U251 cells and the expression level of GFP was detected by fluorescence microscopy. Transgenic mice were produced by microinjection. The genome of the offspring mice was screened by PCR, and GFP expression in the vascular endothelium was detected using immunohistochemistry.</p><p><b>RESULTS</b>Thirteen offspring mice were obtained and 2 of them were positive for GFP in the vascular endothelium as detected by PCR. GFP was detected in the offspring mice both at the embryonic stage and after birth.</p><p><b>CONCLUSIONS</b>The transgenic mice with GFP expression in the vascular endothelium during neovascularization have been successfully established.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Endotélio Vascular / Camundongos Transgênicos / Dados de Sequência Molecular / Sequência de Bases / Neovascularização Fisiológica / Proteínas de Fluorescência Verde / Nestina / Genética / Proteínas de Filamentos Intermediários / Animais Recém-Nascidos Limite: Animais Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2011 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Endotélio Vascular / Camundongos Transgênicos / Dados de Sequência Molecular / Sequência de Bases / Neovascularização Fisiológica / Proteínas de Fluorescência Verde / Nestina / Genética / Proteínas de Filamentos Intermediários / Animais Recém-Nascidos Limite: Animais Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2011 Tipo de documento: Artigo