Development of a time-resolved fluoroimmunoassay for detecting S1 subunit of pertussis toxin and its application / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1509-1512, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-333876
ABSTRACT
<p><b>OBJECTIVE</b>To develop a time-resolved fluoroimmunoassay (TRFIA) for detection of pertussis toxin (PT) S1 subunit for quality control of human PT vaccine.</p><p><b>METHODS</b>A double antibody sandwich one-step method was used to establish the TRFIA for detecting PT S1 subunit in the vaccine.</p><p><b>RESULTS</b>The sensitivity of c peptide analysis reached 2.5 ng/ml without cross-reactions with other antigens. This assay could be used in detecting S1 subunit in the vaccine.</p><p><b>CONCLUSION</b>The TRFIA for detecting PT S1 subunit is simple, sensitive and rapid for quality control of the PT vaccine.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Controle de Qualidade
/
Padrões de Referência
/
Vacina contra Coqueluche
/
Fluorimunoensaio
/
Química
/
Sensibilidade e Especificidade
/
Reações Cruzadas
/
Toxina Pertussis
/
Métodos
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2011
Tipo de documento:
Artigo
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