Effect of metformin on apoptosis of renal cell carcinoma cells in vitro and its mechanisms / 南方医科大学学报
Journal of Southern Medical University
; (12): 1504-1508, 2011.
Article
em Zh
| WPRIM
| ID: wpr-333877
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effect of metformin on the apoptosis of renal cell carcinoma (RCC) cells in vitro and its mechanisms.</p><p><b>METHODS</b>Fluorescent microscopy and flow cytometry were used to examine the changes in the apoptosis of 786-O cells after metformin treatment. The possible signaling molecules involved in this process were analyzed by immunoblot analysis of AMP-activated protein kinase (AMPK) signaling and caspase 9.</p><p><b>RESULTS</b>Metformin induced apoptosis and caspase 9 activation in 786-O cells in low-serum medium but not in normal-serum medium. Metformin also induced AMPK activation in 786-O cells, but this activation was not associated with the cell proliferation inhibition or apoptosis-inducing effect of metformin.</p><p><b>CONCLUSION</b>Metformin can induce apoptosis of RCC cells in vitro, suggesting its potential as a therapeutic agent for RCC.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Patologia
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Farmacologia
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Carcinoma de Células Renais
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Apoptose
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Linhagem Celular Tumoral
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Proliferação de Células
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Caspase 9
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Neoplasias Renais
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Metabolismo
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Metformina
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Ano de publicação:
2011
Tipo de documento:
Article