Dynamic analysis of expression of VEGF and its receptor-2 in mouse model with acute myeloid leukemia / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 852-856, 2009.
Article
em Zh
| WPRIM
| ID: wpr-334010
Biblioteca responsável:
WPRO
ABSTRACT
The objective of study was to explore the role of vascular endothelial growth factor (VEGF) and its receptor-2 in pathogenesis of acute myeloid leukemia. The acute myeloid leukemia model was established on 20 mice with severe combined immunodeficiency (SCID) transplanted by HL-60 cells. The mice were divided into the normal control and test group randomly. The expression of VEGF was detected by enzyme linked immunosorbent assay (ELISA). The expression of VEGFR-2 mRNA was detected by RT-PCR. The results showed that the establishment of acute myeloid leukemia model was succeeded on all SCID mice by HL-60 cell transplantation. The expressions of VEGF and VEGFR-2 mRNAs could be determined on all mice. As compared with the normal control group, the expressions of VEGF and VEGFR-2 mRNAs in the test group significantly increased, but gradually increased during the course of disease. It is concluded that the abnormal expressions of VEGF and VEGFR-2 exist in mice with acute myeloid leukemia, which may be involved in the pathogenesis of AML.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Patologia
/
Medula Óssea
/
Camundongos SCID
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Células HL-60
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Receptor 2 de Fatores de Crescimento do Endotélio Vascular
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Fator A de Crescimento do Endotélio Vascular
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Metabolismo
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Transplante de Neoplasias
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2009
Tipo de documento:
Article