Epitope mapping of hepatitis C virus non-structure protein 5 from a 7 peptide phage library by using immobilized specific monoclonal antibody / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 266-268, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-334232
ABSTRACT
<p><b>OBJECTIVE</b>To screen HCV NS5 mimotopes by using monoclonal antibody and phage peptide library.</p><p><b>METHODS</b>By using HCV NS5 monoclonal antibody as selective molecule, a 7 peptide phage library was biopanned and positive clones were selected by ELISA, competition assay and DNA sequencing.</p><p><b>RESULTS</b>Twelve positive clones were chosen for DNA sequencing. From the experiment and sequencing comparison results, one epitope was confirmed as the mimotope of HCV NS5.</p><p><b>CONCLUSIONS</b>HCV mimotope is obtained by phage peptide library screening. The result provides a new approach for HCV therapy and vaccine development.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Terapêutica
/
Ensaio de Imunoadsorção Enzimática
/
Vacinas Virais
/
Química
/
Sequência de Aminoácidos
/
Hepatite C
/
Proteínas não Estruturais Virais
/
Mapeamento de Epitopos
/
Biblioteca de Peptídeos
/
Alergia e Imunologia
Idioma:
Chinês
Revista:
Chinese Journal of Hepatology
Ano de publicação:
2002
Tipo de documento:
Artigo
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