The study of photochemical immobilization of urease on polyether sulfone film surface / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 13-16, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-334350
ABSTRACT
A new method of using photoactivable ester with azido group was described to immobilize urease on polyether sulfone(PES) film surface. The effects of photoactive enzyme concentration, temperature, pH, irradiation time on the activity of immobilized urease were investigated. Reused times and storage stability were also studied. The results showed that the surface concentration of urease immobilized on PES surface was about 0.33 mg/cm2. When the irradiation time was 5 minutes, the relative activity of immobilized urease was the highest and the activity increased with the increase of the concentration of photoactive urease solution. The optimum pH and temperature of immobilized urease were 7 and 50 degrees C respectively. The relative activity of immobilized urease was stable (50%) after 12 times reused at 50 degrees C.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fotoquímica
/
Polímeros
/
Efeitos da Radiação
/
Sulfonas
/
Urease
/
Estabilidade Enzimática
/
Enzimas Imobilizadas
/
Membranas Artificiais
/
Metabolismo
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2002
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS