Porcine aminopeptidase N is a functional receptor for the PEDV coronavirus / 病毒学报
Chinese Journal of Virology
; (6): 220-225, 2009.
Article
em Zh
| WPRIM
| ID: wpr-334747
Biblioteca responsável:
WPRO
ABSTRACT
Porcine epidemic diarrhea virus (PEDV) causes lethal diarrhea in piglets that leads to great economic losses in East Asia. It was reported that aminopeptidase N (APN) was the receptor for Transmissible gastroenteritis virus (TGEV), Human coronavirus 229E (HCoV-229E) and Feline coronavirus (FeCoV) which all belonged to group I coronavirus including PEDV. It was also confirmed previously that porcine aminopeptidase N (pAPN) could bind to PEDV, and anti-pAPN antibodies could inhibit the combination. To investigate whether pAPN was a receptor for PEDV, we transfected MDCK cells with porcine aminopeptidase (pAPN) cDNA and this enabled non-susceptible cells to support PEDV replication and serial viral propagation. Moreover, the infection was blocked by antibodies against pAPN, implying the critical role of pAPN during virus entry. In addition, immunofluorescence assays for detection of pAPN and PEDV antigens, together with neutralization assays using antibodies against pAPN, further confirmed the correlation between pAPN expression and viral replication in pAPN-transfected MDCK cells. These results indicated that pAPN is a functional receptor for PEDV.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Farmacologia
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Suínos
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Linhagem Celular
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Infecções por Coronavirus
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Antígenos CD13
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Vírus da Diarreia Epidêmica Suína
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Genética
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Metabolismo
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Anticorpos
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Virology
Ano de publicação:
2009
Tipo de documento:
Article