Construcion of a chimeric Japanese encephalits virus/dengue virus-2 / 病毒学报
Chinese Journal of Virology
;
(6): 185-189, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-334753
ABSTRACT
The prM/E gene of DV2 was cloned into the JEV (SA14-14-2 strain) replicon vector which had been constructed previously, and the resulting recombinant plasmid was named pPartialdeltaprM/E. The constructed chimeric clone was linearized and then was transcripted into RNA in vitro. The produced RNA was transfected into BHK-21 cells. Five to seven days later, CPE could be observed on the transfected BHK-21cells, and then the supernatant containing the chimeric virus was collected. The Supernatant was inoculated to BHK-1 cells and C6/36 cells, respectively. CPE could be observed about 4 days post the infection of C6/36cell with the chimeric virus. The results from RT-PCR, IFA, Western blot showed that the virus contained the chimeric RNA and the envelop protein of DV2. However, the chimeric virus could not be passaged in BHK-21 cell. The successful construction of the infectious clone JE/DEN-2 laid the basis for the further research of the DV vaccine.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Recombinação Genética
/
Linhagem Celular
/
Western Blotting
/
Vírus Reordenados
/
Vírus da Encefalite Japonesa (Subgrupo)
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Vírus da Dengue
/
Vetores Genéticos
/
Genética
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Virology
Ano de publicação:
2009
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS