Cloning and expression of single-chain Fv antibodies against H5N1 Avian influenza virus hemagglutinin / 病毒学报
Chinese Journal of Virology
;
(6): 63-67, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-334761
ABSTRACT
To construct Fv antibodies against H5N1 Avian influenza virus hemagglutinin,extracted mRNA from B lymphoblastoid cell lines secreting anti-HA antibodies was used and the VH and VL genes were amplified by RT-PCR and linked together by splicing overlap extension (SOE) with (Gly4 Ser)3 linker. The recombinant plasmid was then transformed to E. coli BL21(DE3) and sequence analysis indicated the total length of scFv was 714 bp and the expression of Fv was validated by PAGE and Western blot.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Proteínas Virais
/
Virologia
/
Aves
/
Proteínas Recombinantes de Fusão
/
Região Variável de Imunoglobulina
/
Regulação da Expressão Gênica
/
Clonagem Molecular
/
Cadeias Pesadas de Imunoglobulinas
/
Cadeias Leves de Imunoglobulina
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Virology
Ano de publicação:
2009
Tipo de documento:
Artigo
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