Rescue of chimeric rabies virus expressing green fluorescent protein / 病毒学报
Chinese Journal of Virology
;
(6): 401-403, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-334788
ABSTRACT
Green fluorescent protein (GFP) gene was inserted into the pseudogene (psi) region of genome of rabies virus rHep-Flury strain, and a recombinant rabies virus carrying GFP, designated as HEP-GFP, was rescued by reverse genetics system. It was demonstrated that green fluorescent protein could be expressed in the chimeric virus after 5 passages in BHK-21 cell line. The research indicated that the pseudogene (psi) region in the genome of rHEP-Flury strain, as an independent functional unit in the process of virus assembly, could independently carry and express exogenous genes.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Vírus da Raiva
/
Proteínas Recombinantes
/
Linhagem Celular
/
Proteínas de Fluorescência Verde
/
Genética
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Virology
Ano de publicação:
2008
Tipo de documento:
Artigo
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