Isolation and genomic sequence analysis of porcine transmissible gastroenteritis virus / 病毒学报
Chinese Journal of Virology
;
(6): 364-368, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-334794
ABSTRACT
A transmissible gastroenteritis virus strain was isolated from suspect samples in Sichuan province and identified by ST cell culture, direct fluorescent antibody test (FA), neutralization test (NT), TME examination and some other methods, then it was named SC-Y. The isolated strain could produce obvious cytopathic effects (CPE), The TCID50 was 10(-3.664)/0.05 mL, The neutralization index is 52.5. cDNA fragments covering the complete genome were amplified by the long reverse transcription PCR. The amplified fragments were further cloned and sequenced. The genome of SC-Y strain was assembled by BioEdit. The length of complete genome was 28590 nucletides, and was composed of 7 ORFs, which was flanked by untranslated regions (UTRs) with 315 bases at the 5'-end and 277 bases at the 3'-end. Phylogenetic analysis based on genome suggested that SC-Y might belong to same subgroup with Purdue strain.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Suínos
/
DNA Viral
/
Testes de Neutralização
/
Sequência de Bases
/
Microscopia Eletrônica
/
Química
/
Classificação
/
Vírus da Gastroenterite Transmissível
/
Técnica Direta de Fluorescência para Anticorpo
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Virology
Ano de publicação:
2008
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS