Codon optimization of the H5N1 influenza virus HA gene gets high expression in mammalian cells / 病毒学报
Chinese Journal of Virology
;
(6): 101-105, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-334839
ABSTRACT
In order to improve the expression of human avian influenza virus hemagglutinin (HA) and meet the pandemic influenza vaccine needs, we optimized and synthesized the whole length of HA gene of H5N1 (A/Anhui/1/2005) influenza virus in accordance with the human's codon preference, and inserted it to the eukaryotic expression vector pDC315 to construct a eukaryotic expression vector pDC315-Mod. HA. This plasmid and the eukaryotic expression vector pDC315-Wt. HA containing wild HA gene were transfected into 293T cells respectively to compare the expression of HA protein. The results showed that according to the comparison and identification by indirect immunofluorescence assay and Western blot test, the expression of HA protein in 293T cells was significantly improved after codon optimization. This laid a foundation for pandemic influenza vaccine research.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
Códon
/
Linhagem Celular
/
Western Blotting
/
Técnica Indireta de Fluorescência para Anticorpo
/
Glicoproteínas de Hemaglutininação de Vírus da Influenza
/
Virus da Influenza A Subtipo H5N1
/
Genética
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Virology
Ano de publicação:
2008
Tipo de documento:
Artigo
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