Cloning NS1 gene of H5N1 avian influenza virus and apoptosis induced by it in human pulmonary carcinoma cell line A549 / 病毒学报
Chinese Journal of Virology
;
(6): 360-365, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-334882
ABSTRACT
The NS1 gene of the H5N1 subtype avian influenza virus was amplified by RT-PCR, and the am-plified product was cloned into the eukaryotic expression vector pCMV-Myc, then it was transfected into A549 cells. After 48 h, the expression of NS1 was detected by Western blot. Fluorescence and electron microscopy and flow cytometry showed that the NS1 gene of H5N1 avian influenza virus could induce apop-tosis in human pulmonary carcinoma cell line A549.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Fisiologia
/
Virulência
/
Clonagem Molecular
/
Proteínas não Estruturais Virais
/
Apoptose
/
Anexina A5
/
Linhagem Celular Tumoral
/
Virus da Influenza A Subtipo H5N1
/
Genética
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Virology
Ano de publicação:
2007
Tipo de documento:
Artigo
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