Construction and identification of tetracycline-inducible rat Smad7 eukaryotic expression vector / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1313-1315, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-334934
ABSTRACT
<p><b>OBJECTIVE</b>To construct a tetracycline-inducible eukaryotic expression vector of rat Smad7.</p><p><b>METHODS</b>The total RNA was extracted from normal rat kidney with Trizol agent. Rat Smad7 cDNA fragment was cloned by RT-PCR, and was inserted into the restriction site between Nhe I and Hind III of the inducible eukaryotic expression vector pBI-L by tetracycline. pBI-L-Smad7 was constructed by digestion and ligation, and detected by restriction endonuclease digestion and sequencing.</p><p><b>RESULTS</b>The recombinant eukaryotic expression vector pBI-L-Smad7 was constructed correctly as confirmed by restriction endonuclease digestion and sequencing. The fragment of pBI-L-Smad7 digested with restriction endonucleases and the sequence of inserted Smad7 cDNA were consistent with the results of theoretical analysis.</p><p><b>CONCLUSION</b>The tetracycline- inducible eukaryotic expression vector of rat Smad7, pBI-L-Smad7, is constructed successfully, which may facilitate further clinical study of Smad7 gene therapy for tissue and organ fibrosis.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Tetraciclina
/
Terapia Genética
/
Expressão Gênica
/
Clonagem Molecular
/
Ratos Sprague-Dawley
/
DNA Complementar
/
Proteína Smad7
/
Células Eucarióticas
/
Vetores Genéticos
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2006
Tipo de documento:
Artigo
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