Labeling of CDTPA-dianhydride-coupled CD45 monoclonal antibody with yttrium-90 / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1230-1232, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-334952
ABSTRACT
<p><b>OBJECTIVE</b>To explore the methods for labeling CDTPA-coupled CD45 monoclonal antibody (mAb) with yttrium-90 ((90)Y) for potential acute myeloid therapy.</p><p><b>METHODS</b>CD45 mAb was labeled with (90)Y by CDTPA and the labeling rate, radiochemical purity, final specific activity, and immunological activity of the mAb were detected.</p><p><b>RESULTS</b>With the optimal molar ratio of CDTPA/Ab at 201, the labeling rate was 95%, radiochemical purity 99.8%, and final specific activity 1.9 mCi/mg. This conjugate was stable in vitro with comparable immunological activity in comparison with unlabeled CD45 mAb.</p><p><b>CONCLUSION</b>(90)Y-CDTPA-CD45 mAb possesses good properties as an ideal targetting therapeutic agent for acute leukemia.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Radioisótopos de Ítrio
/
Química
/
Antígenos Comuns de Leucócito
/
Imunoconjugados
/
Compostos Radiofarmacêuticos
/
Ácido Pentético
/
Alergia e Imunologia
/
Marcação por Isótopo
/
Anidridos
/
Métodos
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2006
Tipo de documento:
Artigo
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