Effects of Saponin B from Anemarrhena asphodeloides Bunge on tau hyperphosphorylation induced by beta-amyloid peptide (25-35) in rats / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the changes in the expressions of p53, DKK (the inhibitor of Wnt pathway) and phosphorylated tau in rat bilateral hippocampus after beta-amyloid peptide (beta-AP) (25-35) injection, and observe the effect of saponin B from Anemarrhena asphodeloides Bunge (SAaB) in this model.</p><p><b>METHODS</b>After bilateral injection of beta-AP (25-35) into the hippocampus of rats, RT-PCR was performed for observing the changes in p53 and DKK mRNA expressions and immunochemistry carried out to detect the changes in phosphorylated tau protein.</p><p><b>RESULTS</b>RT-PCR showed increased p53 and DKK mRNA expression and immunochemistry revealed increased phosphorylated tau-positive cells in rat hippocampus after beta-AP (25-35) injection, and administration of SAaB significantly ameliorated these changes.</p><p><b>CONCLUSION</b>SAaB can significantly ameliorate beta-AP-induced tau hyperphosphorylation by inhibiting increased p53 and DKK mRNA expressions in response to beta-AP injection.</p>