Screening of peptide inhibitors of acetylcholinesterase from 12-mer random phage display peptide library / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To screen the peptide inhibitor of acetylcholinesterase (AChE) from 12-mer random phage display peptide library.</p><p><b>METHODS</b>Human AChE was used as the target to screen its binding peptides from 12-mer random phage display peptide library. The positive phage clones were isolated after three rounds of biopanning followed then by sequence analysis and their activity evaluation.</p><p><b>RESULTS</b>Six positive phage clones binding to human AChE were obtained, and 4 of them sharing the conservative sequence W(S/P)HY inhibited the enzyme activity of AChE.</p><p><b>CONCLUSION</b>Acquisition of AChE inhibitor from phage display library provides clues for designing peptide inhibitors of AChE.</p>