Your browser doesn't support javascript.
loading
As2O3-induced permeability transition pore opening in mitochondria depends on Ca2+ / 南方医科大学学报
Journal of Southern Medical University ; (12): 1030-1033, 2006.
Artigo em Chinês | WPRIM | ID: wpr-335000
ABSTRACT
<p><b>OBJECTIVE</b>To explore the mechanism of arsenite-induced permeability transition pore (PTP) opening and the role of Ca(2+). in As(2)O(3)-induced PTP opening.</p><p><b>METHOD</b>The mitochondria were prepared from Wistar rat liver and mitochondrial swelling was assessed spectrophotometrically at 540 nm to evaluate PTP opening. The membrane potential of the mitochondria was measured with fluorescence spectrophotometry.</p><p><b>RESULTS</b>PTP opening was induced with 10 micromol/L As(2)O(3) in the presence of 10 micromol/L Ca(2+), and the absorbance at 540 nm of the mitochondria did not decrease in response to exclusive treatment with 10 micromol/L As(2)O(3), or to 10 micromol/L As(2)O(3) plus 10 micromol/L Ca(2+) treatment with 0.5 mmol/L EGTA pretreatment. Treatment with As(2)O(3) at 0, 5, 10 and 20 micromol/L in the presence of 50, 20, 10 and 5 micromol/L Ca(2+), respectively, resulted in decreased absorbance at 540 nm of the mitochondria.</p><p><b>CONCLUSION</b>Ca(2+) mediates As(2)O(3)-induced PTP opening. As(2)O(3) lowers Ca(2+) threshold necessary for eliciting PTP opening and thereby regulates cell apoptosis.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Óxidos / Farmacologia / Fisiologia / Arsenicais / Mitocôndrias Hepáticas / Cálcio / Ratos Wistar / Proteínas de Transporte da Membrana Mitocondrial / Relação Dose-Resposta a Droga / Sinergismo Farmacológico Limite: Animais Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2006 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Óxidos / Farmacologia / Fisiologia / Arsenicais / Mitocôndrias Hepáticas / Cálcio / Ratos Wistar / Proteínas de Transporte da Membrana Mitocondrial / Relação Dose-Resposta a Droga / Sinergismo Farmacológico Limite: Animais Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2006 Tipo de documento: Artigo