Isolation and characterization of side population cells in human gastric cancer cell line BGC-823 / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 264-268, 2012.
Article
em Zh
| WPRIM
| ID: wpr-335299
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>Isolate and characterize the side population (SP) cells with potency of stem cells from human gastric carcinoma cell line BGC-823.</p><p><b>METHODS</b>SP and non-SP cells were sorted from BGC-823 cells by fluorescence-activated cell sorting (FACS) using Hoechst33342 staining. The tumorigenic ability of the SP cells was assessed by in vivo transplantation into non-obese diabetic/severe combined immunodeficiency mice.</p><p><b>RESULTS</b>SP cells were isolated from BGC-823 cells in a proportion of 0.9% to 2.1% with respect to the whole cell population. The colony formation assay showed that the colony formation rate of the SP cells was significantly higher than that of the non-SP cells (72.56% vs. 49.00%, P < 0.01). The drug sensitivity test showed that the SP cells showed stronger drug resistance to 5-Fu than the non-SP cells. The in vivo transplantation of SP cells in mice showed that the tumor weight was (0.176 ± 0.034) g, significantly higher than that of non-SP cells (0.045 ± 0.046) g (P < 0.01).</p><p><b>CONCLUSIONS</b>The results of this study indicate the existence of cancer stem-like SP cells in the human gastric cancer line BGC-823 cells. Further characterization of this SP cell population may provide new insights for diagnosis and treatment of gastric cancer.</p>
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Assunto principal:
Patologia
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Farmacologia
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Neoplasias Gástricas
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Transplante
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Camundongos SCID
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Camundongos Endogâmicos NOD
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Ensaio de Unidades Formadoras de Colônias
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Resistencia a Medicamentos Antineoplásicos
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Linhagem Celular Tumoral
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Proliferação de Células
Limite:
Animals
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Female
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Humans
Idioma:
Zh
Revista:
Chinese Journal of Oncology
Ano de publicação:
2012
Tipo de documento:
Article