Ginsenosides stimulated the proliferation of mouse spermatogonia involving activation of protein kinase C / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
;
(12): 87-92, 2009.
Artigo
em Inglês
| WPRIM
| ID: wpr-335396
ABSTRACT
The effect of ginsenosides on proliferation of type A spermatogonia was investigated in 7-day-old mice. Spermatogonia were characterized by c-kit expression and cell proliferation was assessed by immunocytochemical demonstration of proliferating cell nuclear antigen (PCNA). After 72-h culture, Sertoli cells formed a confluent monolayer to which numerous spermatogonial colonies attached. Spermatogonia were positive for c-kit staining and showed high proliferating activity by PCNA expression. Ginsenosides (1.0 approximately10 microg/ml) significantly stimulated proliferation of spermatogonia. Activation of protein kinase C (PKC) elicited proliferation of spermatogonia at 10(-8) to 10(-7) mol/L and the PKC inhibitor H(7) inhibited this effect. Likewise, ginsenosides-stimulated spermatogonial proliferation was suppressed by combined treatment of H(7). These results indicate that the proliferating effect of ginsenosides on mouse type A spermatogonia might be mediated by a mechanism involving the PKC signal transduction pathway.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fisiologia
/
Espermatogônias
/
Proteína Quinase C
/
Antígeno Nuclear de Célula em Proliferação
/
Biologia Celular
/
Ginsenosídeos
/
Proliferação de Células
/
Ativação Enzimática
/
Camundongos Endogâmicos ICR
Limite:
Animais
Idioma:
Inglês
Revista:
Journal of Zhejiang University. Science. B
Ano de publicação:
2009
Tipo de documento:
Artigo
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