Inhibitory effect of hydrogen sulfide on cardiac fibroblast proliferation / 生理学报
Acta Physiologica Sinica
; (6): 353-358, 2011.
Article
em Zh
| WPRIM
| ID: wpr-335979
Biblioteca responsável:
WPRO
ABSTRACT
The aim of the present study was to investigate the role of hydrogen sulfide (H(2)S) in the proliferation of neonatal rat cardiac fibroblasts (NRCFs). Proliferation of NRCFs was induced by the presence of fetal bovine serum (FBS) or angiotensin II (Ang II) at various concentrations. The concentration-dependent effect of NaHS (donor of H(2)S) on NRCFs proliferation was examined. NRCFs proliferation was assessed by 5'-bromo-2'-deoxyuridine (BrdU) incorporation method. Reactive oxygen species (ROS) level was measured using the dye probe, 2', 7'-dichlorofluorescein diacetate (DCFH-DA). The results showed that FBS- or Ang II-induced NRCFs proliferations were inhibited with the treatment of relatively high concentrations of NaHS (5 × 10(-5) mol/L, 1 × 10(-4) mol/L), but FBS-induced proliferation was increased by low concentration of NaHS (1 × 10(-5) mol/L). Two or 6 h of Ang II (1 × 10(-7) mol/L) treatment caused an increase of ROS level in NRCFs, while this increase was inhibited with NaHS (1 × 10(-4) mol/L) treatment. These results suggest that H(2)S is an inhibitor of cardiac fibroblast at a certain concentration range. This inhibitory effect may be mediated by a reduction in intracellular ROS production.
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Índice:
WPRIM
Assunto principal:
Farmacologia
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Angiotensina II
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Células Cultivadas
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Espécies Reativas de Oxigênio
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Ratos Sprague-Dawley
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Biologia Celular
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Depressão Química
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Proliferação de Células
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Fibroblastos
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Sulfeto de Hidrogênio
Limite:
Animals
Idioma:
Zh
Revista:
Acta Physiologica Sinica
Ano de publicação:
2011
Tipo de documento:
Article