Inhibitory effect of a HBV vector plasmid expressing A3C on HBV replication / 南方医科大学学报
Journal of Southern Medical University
;
(12): 2044-2050, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-336025
ABSTRACT
<p><b>OBJECTIVE</b>To observe the inhibitory effect of a replication-defective hepatitis B virus (HBV) vector plasmid expressing A3C on HBV replication in vitro.</p><p><b>METHODS</b>The HBV vector plasmisd pCH-LJ3-A3C and pCH-LJ3-hrGFP expressing A3C and hrGFP were constructed using PCR and gene recombination technique. The two recombinant plasmids were separately cotranfected into HepG2 cells along with the wild-type HBV plasmid pCH-3093. The HBV DNA in the cell cytoplasmic lysates and in the cell culture supernatant was extracted for Southern blotting, and the nucleocapsid-associated HBV DNA were amplified by PCR, cloned and sequenced.</p><p><b>RESULTS</b>pCH-LJ3-A3C showed obvious inhibitory effect on HBV DNA in the cytoplasmic lysates and cell culture supernatant, causing a reduction of the HBV DNA by 31% and 40%, respectively. The pCH-LJ3-A3C plasmid was capable of editing the HBV DNA. Among the 50 sequenced clones, 36 clones had G-A mutations, with a total of 982 such mutations.</p><p><b>CONCLUSION</b>pCH-LJ3-A3C can inhibit the replication of HBV primarily by editing HBV DNA. The pCH-LJ3-A3C plasmid may serve as a new antiviral agent against human HBV infection.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Antivirais
/
Farmacologia
/
Fisiologia
/
Plasmídeos
/
Replicação Viral
/
RNA Mensageiro
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Vírus da Hepatite B
/
Citidina Desaminase
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2009
Tipo de documento:
Artigo
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