Phylogenetic analysis and PCR-restriction fragment length polymophism identification of Salmonella based on grOEL gene sequence / 南方医科大学学报
Journal of Southern Medical University
; (12): 2037-2043, 2009.
Article
em Zh
| WPRIM
| ID: wpr-336027
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the value of grOEL gene sequence in phylogenetic analysis and typing of Salmonella.</p><p><b>METHODS</b>The grOEL gene was amplified by PCR, sequenced and analyzed using Bioedit and DNAstar software. The Salmonella strains were identified using PCR-restriction fragment length polymophism (PCR-RFLP).</p><p><b>RESULTS</b>The conservative and variable regions of grOEL gene of Salmonella serogroup were separately distributed and most of the small mutant regions distributed intermittently among the conservative regions. The phylogenetic tree of Salmonella based on the nucleotides differed from that generated based on the amino acid sequence. O8, O9 and O10 had the closest consanguinity, and 5 patterns were identified by PCR-RFLP.</p><p><b>CONCLUSION</b>The grOEL gene can be used as a genetic marker for phylogenetic analysis of Salmonella and also as a target sequence for Salmonella typing identification.</p>
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Índice:
WPRIM
Assunto principal:
Filogenia
/
Salmonella
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Polimorfismo de Fragmento de Restrição
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DNA Bacteriano
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Reação em Cadeia da Polimerase
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Técnicas de Tipagem Bacteriana
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Classificação
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Análise de Sequência de DNA
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Chaperonina 60
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Genética
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Idioma:
Zh
Revista:
Journal of Southern Medical University
Ano de publicação:
2009
Tipo de documento:
Article