MTT assay for detecting osteosarcoma cell apoptosis / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1899-1901, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-336055
ABSTRACT
<p><b>OBJECTIVE</b>To assess the feasibility of using MTT assay for detecting the apoptosis of osteosarcoma cells following chemotherapy.</p><p><b>METHODS</b>The osteosarcoma cells derived form surgical specimens were cultured in RPMI 1640 medium supplemented with 10% calf serum. Chemotherapeutic agents were administered in the cell culture, and MTT assay was used to observe the cell apoptosis under optical microscope.</p><p><b>RESULTS</b>MTT staining accurately identified apoptosis of the osteosarcoma cells, and the apoptotic cells were easily distinguished from normal cells and dead cells.</p><p><b>CONCLUSION</b>MTT staining is convenient and practical for detecting osteosarcoma cell apoptosis.</p>
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DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Cirurgia Geral
/
Sais de Tetrazólio
/
Tiazóis
/
Neoplasias Ósseas
/
Células Tumorais Cultivadas
/
Protocolos de Quimioterapia Combinada Antineoplásica
/
Osteossarcoma
/
Apoptose
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2009
Tipo de documento:
Artigo
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