Effects of RNA interference against aggrecanase 1 gene on extracellular matrix metabolism of cultured chondrocytes in vitro / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1766-1769, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-336091
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of RNA interference (RNAi)-mediated aggrecanase-1 gene silencing on extracellular matrix metabolism of cultured rat costochondral chondrocytes.</p><p><b>METHODS</b>Rat costochondral chondrocyte monolayers were obtained by microdissection and digestion. The growth and morphological changes of the chondrocytes were observed after RNAi of aggrecanase-1 gene. The mRNA expression of aggrecanase-1 was detected by RT-PCR method, and aggrecan content was determined by Western blotting.</p><p><b>RESULTS</b>The specific inhibition of aggrecanase-1 by RNAi produced no adverse effect on the morphology and growth of the chondrocytes. The mRNA of aggrecanase-1 decreased and aggrecan content increased significantly after transfection of the chondrocytes.</p><p><b>CONCLUSION</b>Inhibition of aggrecanase-1 decreases aggrecan degradation in cultured rat chondrocytes. RNAi technique can be a useful means for studying extracellular matrix metabolism in the cartilage.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pró-Colágeno N-Endopeptidase
/
RNA Mensageiro
/
Transfecção
/
Células Cultivadas
/
Ratos Sprague-Dawley
/
Condrócitos
/
Biologia Celular
/
Interferência de RNA
/
Proteínas ADAM
/
Matriz Extracelular
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2009
Tipo de documento:
Artigo
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