Induction and characterization of induced pluripotent stem (iPS) cells: a review / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 421-430, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-336210
ABSTRACT
The somatic cells can be induced into ES-like stem cells when retrovirally infected the defined transcription factors including Oct4, Sox2, Klf4 and c-Myc. These ES-like cells are named induced pluripotent stem (iPS) cells and this method is called iPS technology. Until the end of 2009, iPS cell lines have been generated in various animal species, such as mouse, human, rhesus monkey, rat and pig. Mouse iPS cells are also used to generate chimera mice and viable mice through the tetraploid complementation. Although iPS cells are extremely similar to ES cells in both morphology and growth features, to generate iPS cells do need the defined culture procedures. Based on the update global iPS technology development and the iPS studies in our laboratory, this paper focused on the establishment of iPS cell lines and improvement of iPS cell culture condition.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fatores de Transcrição
/
Diferenciação Celular
/
Proteínas Proto-Oncogênicas c-myc
/
Técnicas de Cultura de Células
/
Biologia Celular
/
Fatores de Transcrição Kruppel-Like
/
Fator 3 de Transcrição de Octâmero
/
Células-Tronco Embrionárias
/
Fatores de Transcrição SOXB1
/
Células-Tronco Pluripotentes Induzidas
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2010
Tipo de documento:
Artigo
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