Expression and purification of fusion protein CTP-SOD in Pichia pastoris and antioxidant capacity analysis / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 324-329, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-336224
ABSTRACT
Cytoplasmic transduction peptide (CTP) is a newly designed transduction peptide, by which special molecules can be carried out and localized into cytoplasmic compartment. Superoxide dismutase (SOD) is a protein that is difficult to go into cytoplasm. In this study, CTP-SOD fusion gene was amplified from human cDNA by PCR, and the active recombinant protein was successfully expressed in Pichia pastoris. HeLa cells pretreated with CTP-SOD showed a significantly improved survival against the pyrogallol-induced oxidative stress, suggesting CTP-SOD could cross the cell membrane more efficiently and protect cells from oxidative stress.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fisiologia
/
Pichia
/
Superóxido Dismutase
/
Proteínas Recombinantes de Fusão
/
Células HeLa
/
Transdução de Sinais
/
Estresse Oxidativo
/
Citoplasma
/
Peptídeos e Proteínas de Sinalização Intracelular
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2010
Tipo de documento:
Artigo
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