Your browser doesn't support javascript.
loading
Molecular cloning and preliminary analysis of TaNOA in common wheat / 生物工程学报
Chinese Journal of Biotechnology ; (12): 48-56, 2010.
Artigo em Chinês | WPRIM | ID: wpr-336262
ABSTRACT
Nitric oxide (NO) is an important signaling molecule with diverse physiological functions in both animal and plant cells. In this work, we isolated the full-length cDNA and genomic DNA sequences of TaNOA-B1 encoding a putative NO associated (NOA) factor in common wheat. Bioinformatic analysis showed that TaNOA-B1 possessed a similar intron/exon structure as its orthologous genes in Arabidopsis and rice. The amino acid sequence deduced from TaNOA-B1 was more than 60% identitical to those of Arabidopsis and rice NOA1 proteins. The primary structure of TaNOA-B1 contained the zinc finger and P-loop GTPase motifs conserved in Arabidopsis and rice NOA1 proteins. There existed at least three NOA gene members in common wheat, which were mapped to homoeologous group six chromosomes 6A, 6B and 6D, respectively. TaNOA-B1 investigated in this work was located on chromosome 6B. The transcripts of TaNOA members were found mainly in leaves. TaNOA-B1-GFP fusion protein may be located in mitochondria. TaNOA transcript level was up-regulated by abscisic acid (ABA) or NaC1 treatments, indicating that TaNOA might be involved in wheat responses to abiotic stresses.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Proteínas de Plantas / Oryza / Triticum / Dados de Sequência Molecular / Íntrons / Éxons / Sequência de Aminoácidos / Clonagem Molecular / Genes de Plantas / Arabidopsis Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2010 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Proteínas de Plantas / Oryza / Triticum / Dados de Sequência Molecular / Íntrons / Éxons / Sequência de Aminoácidos / Clonagem Molecular / Genes de Plantas / Arabidopsis Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2010 Tipo de documento: Artigo