Study on prohibition of high mobility group chromosomal protein N2 against human oral squamous cell carcinoma in vitro / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 91-95, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-336385
ABSTRACT
<p><b>OBJECTIVE</b>Take human oral squamous cell carcinoma Tca8113 as experimental model, and study the anti oral squamous cell carcinoma activity of high mobility group chromosomal protein N2 (HMGN2) molecule.</p><p><b>METHODS</b>Train a large number of recombinant human HMGN2 expression vector Escherichia coli BL21. HMGN2 was expressed under isopropyl-1-thio-beta-galactopyranoside (IPTG) induction and purified by B-PER GST Fusion Protein Purification Kit. A variety of concentrations HMGN2 were added to cell culture medium, cells were tested by MTT, Hoechst 33342 fluorescence staining, flow cytometry assay and Western-blot.</p><p><b>RESULTS</b>MTT results proved that HMGN2 could significantly inhibit human oral squamous cell carcinoma Tca8113 growth. Hoechst 33342 fluorescence staining, flow cytometry assay test and Western-blot proved HMGN2 could make Tca8113 cells morphological change, make Tca8113 cells block in S period of cell cycle and strongly promote Tca8113 cells to apoptosis.</p><p><b>CONCLUSION</b>HMGN2 can promote apoptosis of oral squamous cell carcinoma cells.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Técnicas In Vitro
/
Proteínas Recombinantes
/
Neoplasias Bucais
/
Proteínas de Grupo de Alta Mobilidade
/
Carcinoma de Células Escamosas
/
Apoptose
/
Proliferação de Células
Limite:
Humanos
Idioma:
Chinês
Revista:
West China Journal of Stomatology
Ano de publicação:
2013
Tipo de documento:
Artigo
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