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Effects of anti-angiopoietin-2 antibody on vascularization of an implanted model of human colonic carcinoma on chick embryo / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery ; (12): 278-280, 2007.
Artigo em Chinês | WPRIM | ID: wpr-336459
ABSTRACT
<p><b>OBJECTIVE</b>To establish an implanted model of human colonic carcinoma on chick embryo, and to study the effects of anti-angiopoietin-2 antibody on its vascularization.</p><p><b>METHODS</b>The human colonic adenocarcinoma cell line HT-29 was transplanted on the chick embryo's chorioallantoic membrane(CAM), and the angiogenesis characteristics were observed by stero-microscope, light microscope and immunohistochemistry. Furthermore, the effects of anti-angiopoietin-2 antibody on angiogenesis and tumor growth were also investigated.</p><p><b>RESULTS</b>Three to seven days after HT-29 cell line was implanted into CAM, tumors grew rapidly and new blood vessels grew toward tumors. Five days after anti-angiopoietin-2 antibody was given, the number of blood vessels in anti-angiopoietin-2 antibody group was significantly down-regulated than that in tumor control group observed by stero-microscope (37.2+/-4.6 vs 56.8+/-7.4, P<0.01), but was up-regulated than that in normal control group (37.2+/-4.6 vs 9.6+/-2.4, P<0.01). Microvessel density(MVD) in anti-angiopoietin-2 antibody group was much lower than that in tumor control group by histological examination (9.6+/-2.4 vs 20.2+/-5.8, P<0.01).</p><p><b>CONCLUSION</b>Angiopoietin-2 antibody is able to inhibit the angiogenesis induced by colorectal cancer cell line HT-29 obviously. The anti-angiopoietin-2 antibody may be potentially useful for clinical treatment of colonic carcinoma.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Neoplasias do Colo / Células HT29 / Angiopoietina-2 / Alergia e Imunologia / Anticorpos Monoclonais / Transplante de Neoplasias / Neovascularização Patológica Tipo de estudo: Estudo prognóstico Limite: Animais / Humanos Idioma: Chinês Revista: Chinese Journal of Gastrointestinal Surgery Ano de publicação: 2007 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Neoplasias do Colo / Células HT29 / Angiopoietina-2 / Alergia e Imunologia / Anticorpos Monoclonais / Transplante de Neoplasias / Neovascularização Patológica Tipo de estudo: Estudo prognóstico Limite: Animais / Humanos Idioma: Chinês Revista: Chinese Journal of Gastrointestinal Surgery Ano de publicação: 2007 Tipo de documento: Artigo