Screening of differentially expressed genes during adipocyte differentiation by suppression subtractive hybridization technique / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 267-273, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-336797
ABSTRACT
<p><b>OBJECTIVE</b>To screening differentially expressed genes related to adipocyte differentiation.</p><p><b>METHODS</b>Total RNA extracted from the preadipocyte cell line SW872 was taken as the Driver and the total RNA from the differentiated adipocytes SW872 as the Tester. Suppression subtractive hybridization (SSH) was used to isolate the cDNA fragments of differentially expressed genes. The products of SSH were inserted into pGM-T vector to establish the subtractive library. The library was amplified through E.coli transformation and positive clones of the transformants were screened. Positive clones were sequenced. Nucleic acid similarity was subsequently analyzed by comparing with the data from GenBank.</p><p><b>RESULTS</b>There were 135 white clones in the cDNA library, 64 positive clones were chosen randomly and sequenced and similarity search revealed 34 genes which expressed differentially in adipocyte differentiation.</p><p><b>CONCLUSION</b>The subtracted cDNA library for differentially expressed in adipocyte differentiation has been successfully constructed and the interesting candidate genes related to adipocyte differentiation have been identified.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Transformação Bacteriana
/
Biblioteca Gênica
/
Diferenciação Celular
/
Linhagem Celular
/
Clonagem Molecular
/
Adipócitos
/
Perfilação da Expressão Gênica
/
Biologia Celular
/
Vetores Genéticos
/
Genética
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2012
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS