Identification and tissue localization of intermediate filament protein in Angiostrongylus cantonensis / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1141-1144, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-337311
ABSTRACT
<p><b>OBJECTIVE</b>To identify the type of the intermediate filament (IF) protein of Angiostrongylus cantonensis and analyze its tissue localization.</p><p><b>METHODS</b>Recombinant pET-IF of antigen IF was expressed in E.coli with IPTG induction, and the expression products were purified by His.Bind column and identified for determining the type of the IF protein by Western blotting. Anti-IF antibody was prepared by multi-spot subcutaneous injection into mouse and used to detect the tissue slices of A. cantonensis by immunohistochemical analysis.</p><p><b>RESULTS</b>The antigen IF were correctly expressed and purified, and identified as a keratin located in the intestine wall and cytoplusma.</p><p><b>CONCLUSION</b>The antigen IF is distributed in the intestine wall of A. cantonensis.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Núcleo Celular
/
Classificação
/
Angiostrongylus cantonensis
/
Transporte Proteico
/
Biologia Celular
/
Eletroforese em Gel de Poliacrilamida
/
Genética
/
Proteínas de Filamentos Intermediários
/
Metabolismo
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2007
Tipo de documento:
Artigo
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