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HL-60 cell apoptosis induced by bortezomib alone or in combination with arsenic trioxide in vitro / 南方医科大学学报
Journal of Southern Medical University ; (12): 1022-1025, 2007.
Artigo em Chinês | WPRIM | ID: wpr-337332
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of bortezomib alone and in combination with arsenic trioxide on apoptosis of HL-60 cells.</p><p><b>METHODS</b>HL-60 cells were treated with bortezomib alone or in combination with arsenic trioxide for 12 to 48 h and the cell proliferation was analyzed with MTT assay, and cell apoptosis detected by DNA gel electrophoresis, fluorescence microscopy and flow cytometry.</p><p><b>RESULTS</b>At the concentrations of 10 to 50 nmol/L, bortezomib effectively inhibited HL-60 cell proliferation, and induced cell apoptosis. A 12-hour bortezomib treatment at 10 nmol/L was sufficient to induce cell apoptosis, and prolonged treatment or increased concentration significantly increased HL-60 cell apoptotic rate. Combined treatment of the cells with bortezomib (10 nmol/L) and arsenic trioxide (15 micromol/L) resulted in an even higher cell apoptosis rate than that induced by the respective agent alone.</p><p><b>CONCLUSION</b>Bortezomib can induce HL-60 cell apoptosis in a time- and dose-dependent manner, and a synergistic effect can be observed of bortezomib and arsenic trioxide in apoptosis induction.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Óxidos / Farmacologia / Arsenicais / Pirazinas / Fatores de Tempo / Ácidos Borônicos / Sobrevivência Celular / Apoptose / Células HL-60 / Proliferação de Células Limite: Animais / Humanos Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2007 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Óxidos / Farmacologia / Arsenicais / Pirazinas / Fatores de Tempo / Ácidos Borônicos / Sobrevivência Celular / Apoptose / Células HL-60 / Proliferação de Células Limite: Animais / Humanos Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2007 Tipo de documento: Artigo