Cloning and functional study of a novel aromatic-ring-hydroxylating dioxygenase gene / 南方医科大学学报
Journal of Southern Medical University
; (12): 717-719, 2007.
Article
em En
| WPRIM
| ID: wpr-337357
Biblioteca responsável:
WPRO
ABSTRACT
The aromatic-ring-hydroxylating dioxygenase is a key enzyme that initiates the biodegradation of polycyclic aromatic hydrocarbons in bacteria. In the present study, a novel dioxygenase sequence was cloned from Terrabacter sp. FLO using a genome walking method. The dioxygenase was cloned into pET17 and actively expressed in E.coli BL21 (DE3) in co-expression with electron transfer chain proteins. The recombinant dioxygenase was found to transform phenanthrene, fluorene, pyrene and fluoranthene into the cis-dihydrodiol metabolites.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Fenantrenos
/
Hidrocarbonetos Policíclicos Aromáticos
/
Pirenos
/
Proteínas de Bactérias
/
Biodegradação Ambiental
/
Proteínas Recombinantes
/
Actinomycetales
/
Dados de Sequência Molecular
/
Clonagem Molecular
/
Dioxigenases
Idioma:
En
Revista:
Journal of Southern Medical University
Ano de publicação:
2007
Tipo de documento:
Article