Transcriptome analysis of bioenergy plant Miscanthus sinensis Anderss by RNA-Seq / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1437-1448, 2015.
Article
em Zh
| WPRIM
| ID: wpr-337477
Biblioteca responsável:
WPRO
ABSTRACT
Miscanthus sinensis Anderss is a perennial C4-grass. It is a promising bioenergy plant, which has been proposed as general feedstock for biomass and lignocellulosic biofuel production. In this study, the flower and leaf buds transcriptomes of Miscanthus sinensis Anderss were sequenced by the platform of Illumina HiSeq 2000. In total 98 326 Unigenes were generated by de novo assembly with an average length of 822 bp and N50 of 1 023 bp. Based on the NR, NT, Swiss-Prot, KEGG, GO and COG databases (Evalue < le-5), 74 134 (75.40%) Unigenes were annotated. A total of 45 507 Unigenes were mapped into different GO terms. In KEGG pathways identification, 36 710 sequences were assigned to 128 KEGG pathways. Sorghum bicolor (37 731, 60.86%), Zea mays (16 258, 26.22%), and Oryza sativa (3 065, 4.94%) showed high similarity to Miscanthus sinensis Anderss. And 24 photosynthesis-related enzyme genes were identified. The result provides a foundation for further characterizing the functional genes in Miscanthus sinensis Anderss.
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Índice:
WPRIM
Assunto principal:
Análise de Sequência de RNA
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Genes de Plantas
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RNA de Plantas
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Perfilação da Expressão Gênica
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Biocombustíveis
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Transcriptoma
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Genética
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Poaceae
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Metabolismo
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2015
Tipo de documento:
Article