Breeding of high-producing LI-F lipopeptide Paenibacillus polymyxa by protoplast fusion and differential expression analysis of fusion strains / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1401-1407, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-337480
ABSTRACT
Auxotrophic strains of N1-37 (Phe-) and N2-27 (His-), screened from mutations of Paenibacillus polymyxa JSa-9 previously, were used as the parent strains to screen high-producing LI-F antibacterial lipopeptide fusion strain through protoplast fusion with polyethylene glycol as a promote agent. Fusion strain F5-15 was obtained. Then the product of LI-F antibacterial lipopeptide was quantified by HPLC, and the difference of expression of the key genes of lipopeptide synthase between wild strain JSa-9 and the fusion strain was analyzed by real-time PCR. LI-F antibacterial lipopeptide yield of the fusion strain F5-15 was 3.1-fold of the original strain JSa9's, and the expression levels of the target genes were 10.48, 2.48, 2.1 and 11.8 fold of the initial strain JSa-9, respectively.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Protoplastos
/
Cromatografia Líquida de Alta Pressão
/
Lipopeptídeos
/
Paenibacillus
/
Reação em Cadeia da Polimerase em Tempo Real
/
Metabolismo
/
Antibacterianos
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2015
Tipo de documento:
Artigo
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