Dendritic cells cultured with human umbilical cord serum instead of fetal calf serum / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 236-239, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-337602
ABSTRACT
To investigate whether the dendritic cells (DC) could grow up in cultural system with umbilical cord serum (UCS), the UCS was used in the culture instead of fetal calf serum. The phenotype of dendritic cells was detected by flow cytometry and the antigen presenting ability of DC in allo-MLR was measured by MTT assay. The results showed that DC grown in UCS (UCS-DC) had higher expression rate of CD86, CD83 and HLA-DR than that in grown in FCS (FCS-DC). (P < 0.05), and their expression of CD1a was lower than that of FCS-DC. The ability to induce T cell proliferation had no difference between UCS-DC and FCS-DC. It is suggested that dendritic cells with more mature phenotype had been produced in the medium containing UCS than those in the medium containing FCS, and UCS-DC possessed potent ability to stimulate proliferation of allogeneic T cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fisiologia
/
Células Dendríticas
/
Imunoglobulinas
/
Glicoproteínas de Membrana
/
Antígenos HLA-DR
/
Antígenos CD
/
Divisão Celular
/
Técnicas de Cultura de Células
/
Meios de Cultura
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2002
Tipo de documento:
Artigo
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