Study on clonal proliferation of TCR Vbeta subfamily T cells induced by AML-M(2a) cells in vivo and in vitro / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 299-302, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-337634
ABSTRACT
To observe the expression and clonal expansion of TCR Vbeta subfamily T cells induced by AML-M(2a) cells in vivo and in vitro, complementary determining region 3 (CDR3) of TCR beta with variable region genes was amplified by using RT-PCR in both peripheral blood mononuclear cells (PBMNC) and T cells from mixed lymphocyte and tumor culture (MLTC) from four AML-M(2a) patients. The positive products were further analyzed to identify the clonality of T cells by genescan. The results showed that the similarity distribution of TCR Vbeta subfamily T cells was found Vbeta in PBMNC and MLTC. One or two clonality expansions of T cells could be found in predominant TCR Vbeta subfamily T cells induced by A ML-M2a cells from 3 cases in vivo and in vitro. It was concluded that clonal expansion of TCR Vbeta subfamily T cells stimulated selectively by AML-M(2a) cells may be a specific immune response of patient's T cells to AML-M(2a) cells associated antigen. The clonal proliferation of TCR Vbeta subfamily T cells were affected somewhat by environmental difference in vivo and in vitro.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ativação Linfocitária
/
Linfócitos T
/
Rearranjo Gênico do Linfócito T
/
Leucemia Mieloide Aguda
/
Genes Codificadores da Cadeia beta de Receptores de Linfócitos T
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Alergia e Imunologia
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2002
Tipo de documento:
Artigo
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