Mesenchymal stem cells suppress allogeneic T cell responses by secretion of TGF-beta1 / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 285-288, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-337637
ABSTRACT
To investigate the mechanisms underlying the issue that bone marrow mesenchymal stem cells (MSC) could trigger low responsiveness of allogeneic T lymphocytes against alloantigens, phenotypes of T cells were analysed with flow cytometry before and after coculture of allogeneic T lymphocytes with MSC. Further, expression of cytokines including IL-4, IFN-gamma and TGF-beta1 was evaluated by RT-PCR and ELISA as well. The results showed that CD8(+) subpopulation was increased and CD25(+) subset was decreased in proportion after coculture of allogeneic T lymphocytes with MSC for 2 weeks. RT-PCR assay showed that MSC expressed TGF-beta1 but not IL-4 and IFN-gamma, and the result was further proved by ELISA assay showing that the secretion of TGF-beta1 reached to 1 ng/ml in 72 hours. It was concluded that allogeneic MSC suppressed T cells activation by alteration of T cell subpopulations. The suppressive effect was at least in part due to the secretion of TGF-beta1. The results indicate that MSC pretreatment might be useful in the prevention of GVHD in HLA-mismatched bone marrow transplantation and further donors for hematopoietic stem cells could be selected from greater potentials.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Células-Tronco
/
Células da Medula Óssea
/
Ensaio de Imunoadsorção Enzimática
/
Linfócitos T
/
Fator de Crescimento Transformador beta
/
Transplante de Medula Óssea
/
Teste de Cultura Mista de Linfócitos
/
Secreções Corporais
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2002
Tipo de documento:
Artigo
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