Effect of p53 gene on telomerase activity and hTERT gene expression in HL-60 cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 527-530, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-337681
ABSTRACT
To investigate the change of telomerase activity and human telomerase reverse transcriptase (hTERT) gene expression in HL-60 cells transfected with wild type p53 gene, wild type p53 gene was introduced into HL-60 cells by Lipofectin transfection. Apoptosis was analyzed by TUNEL assay. Telomerase activity and the level of hTERT mRNA were detected by telomeric repeat amplification protocol (TRAP)-ELISA and RT-PCR, respectively. The results showed that the apoptotic rate of HL-60-pN53cG cells was 8.3% and 21.0% respectively after cultured at 32.5 degrees C for 24 h and 72 h. The level of hTERT mRNA was decreased to 68.4% and 55.8% and telomerase activity to 27.3% and 8.9% of control value in HL-60-pN53cG cells at the same points. In conclusions, hTERT mRNA and telomerase activity were down-regulated in HL-60 cells transfected with p53 gene. This may be one of mechanisms of apoptosis induced by wild type p53 gene.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
RNA Mensageiro
/
Expressão Gênica
/
Genes p53
/
Apoptose
/
Telomerase
/
Células HL-60
/
Proteínas de Ligação a DNA
/
Genética
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2002
Tipo de documento:
Artigo
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