The technique of simultaneous recording calcium transients and spontaneous transient outward currents in arterial smooth muscle cells / 生理学报
Acta Physiologica Sinica
;
(6): 269-274, 2010.
Artigo
em Inglês
| WPRIM
| ID: wpr-337749
ABSTRACT
Laser scanning confocal microscopy (LSCM) and whole-cell perforated patch-clamp techniques were combined to study simultaneously the changes of intracellular signal molecules and membrane currents. Intracellular calcium transients and spontaneous transient outward currents (STOCs) were recorded simultaneously in freshly isolated mouse cerebral artery smooth muscle cells. The cells loaded with fluo-4/AM were scanned with the confocal line-scan mode. Triggering voltage pulses derived from an EPC-10 patch clamp amplifier triggered the confocal line scan. The results showed that STOCs and intracellular calcium transients could be simultaneously recorded in the same cell. This technique will be useful in studies of diseases caused by impairments of intracellular Ca(2+) signaling and related ionic channel activities, or vice versa.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Artérias Cerebrais
/
Técnicas de Patch-Clamp
/
Sinalização do Cálcio
/
Biologia Celular
/
Miócitos de Músculo Liso
Limite:
Animais
Idioma:
Inglês
Revista:
Acta Physiologica Sinica
Ano de publicação:
2010
Tipo de documento:
Artigo
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