HRM identification of Chinese medicinal materials Mutong / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2304-2308, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-337940
ABSTRACT
High resolution melting (HRM), an important technology for genotyping and mutation scanning, has broad prospects in the authentification of traditional Chinese medicine. This paper selected universal trnH-psbA primers and used HRM to establish a new methods for identification of Akebia herbs. PCR was conduct at the annealing temperature of 58 degrees C and 35 cycles. The range of the DNA template concentration, the primer concentration and the Mg2+ ion concentration were further analyzed. The results showed the Tm values of Caulis Akebiae was (81.84 ± 0.16), (85.28 ± 0.16) degrees C and Caulis Clematidis Armandii was (83.22 ± 0.19) degrees C and Caulis Aristolochiae manshuriensis was (81.67 ± 0.14) degrees C, (84.24 ± 0.10) degrees C with 5-125 mg - L-' DNA template, 0.4 μmol x L(-1) primer, 2.0 mmol x L(-1) Mg2+. This method can achieve the authentification of Akebia herbs and is simple, fast, high-throughput, visual.
Texto completo:
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Química
/
Técnicas de Química Analítica
/
Classificação
/
DNA de Plantas
/
Magnoliopsida
/
Temperatura de Transição
/
Genética
/
Genótipo
/
Métodos
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2015
Tipo de documento:
Artigo
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