Activation of Nuclear Factor Kappa B by Inducers in Gestational Tissues at Term / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
;
: 1736-1740, 2003.
Artigo
em Coreano
| WPRIM
| ID: wpr-33835
ABSTRACT
OBJECTIVE:
Nuclear factor kappa B (NF-kappa B) is a transcriptional factor in the expression of cyclooxygenase 2 (COX-2), the key enzyme in production of prostaglandins. The purpose of this study was to investigate the activation of NF-kappa B in human gestational tissues obtained from term pregnant women by various inducers.METHODS:
Myometrium, chorion, and amnion were collected during cesarean section from term pregnant women not in labor. Cells from gestational tissues were isolated and cultured with Interleukin-1beta (IL-1beta), Tumor Necrosis Factor-alpha (TNF-alpha) or Lipopolysaccharide (LPS). The activation of NF-kappa B in cells of each tissues was measured by luciferase assay.RESULTS:
Luciferase activity analysis showed significantly higher activity of NF-kappa B in myometrial cells treated with LPS, in chorion cells treated with IL-1beta and TNF-alpha, and in amnion cells treated with IL-1beta and LPS than control.CONCLUSION:
In cells of gestational tissue at term pregnancy, the activation of NF-kappa B is cell-specific and various according to inducers.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Cesárea
/
Prostaglandinas
/
Córion
/
Citocinas
/
NF-kappa B
/
Fator de Necrose Tumoral alfa
/
Gestantes
/
Ciclo-Oxigenase 2
/
Interleucina-1beta
/
Âmnio
Limite:
Animais
/
Feminino
/
Humanos
/
Gravidez
Idioma:
Coreano
Revista:
Korean Journal of Obstetrics and Gynecology
Ano de publicação:
2003
Tipo de documento:
Artigo
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