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Detection of neural stem cells function in rats with traumatic brain injury by manganese-enhanced magnetic resonance imaging / 中华医学杂志(英文版)
Chinese Medical Journal ; (24): 1848-1853, 2011.
Artigo em Inglês | WPRIM | ID: wpr-338576
ABSTRACT
<p><b>BACKGROUND</b>Previously we had successfully tracked adult human neural stem cells (NSCs) labeled with superparamagnetic iron oxide particles (SPIOs) in host human brain after transplantation in vivo non-invasively by magnetic resonance imaging (MRI). However, the function of the transplanted NSCs could not be evaluated by the method. In the study, we applied manganese-enhanced MRI (ME-MRI) to detect NSCs function after implantation in brain of rats with traumatic brain injury (TBI) in vivo.</p><p><b>METHODS</b>Totally 40 TBI rats were randomly divided into 4 groups with 10 rats in each group. In group 1, the TBI rats did not receive NSCs transplantation. MnCl2·4H2O was intravenously injected, hyperosmolar mannitol was delivered to disrupt rightside blood brain barrier, and its contralateral forepaw was electrically stimulated. In group 2, the TBI rats received NSCs (labeled with SPIO) transplantation, and the ME-MRI procedure was same to group 1. In group 3, the TBI rats received NSCs (labeled with SPIO) transplantation, and the ME-MRI procedure was same to group 1, but diltiazem was introduced during the electrical stimulation period. In group 4, the TBI rats received phosphate buffered saline (PBS) injection, and the ME-MRI procedure was same to group 1.</p><p><b>RESULTS</b>Hyperintense signals were detected by ME-MRI in the cortex areas associated with somatosensory in TBI rats of group 2. These signals, which could not be induced in TBI rats of groups 1 and 4, disappeared when diltiazem was introduced in TBI rats of group 3.</p><p><b>CONCLUSION</b>In this initial study, we mapped implanted NSCs activity and its functional participation within local brain area in TBI rats by ME-MRI technique, paving the way for further pre-clinical research.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fisiologia / Cirurgia Geral / Transplante / Lesões Encefálicas / Imageamento por Ressonância Magnética / Aumento da Imagem / Movimento Celular / Ratos Sprague-Dawley / Células-Tronco Neurais / Manganês Tipo de estudo: Estudo diagnóstico Limite: Animais Idioma: Inglês Revista: Chinese Medical Journal Ano de publicação: 2011 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fisiologia / Cirurgia Geral / Transplante / Lesões Encefálicas / Imageamento por Ressonância Magnética / Aumento da Imagem / Movimento Celular / Ratos Sprague-Dawley / Células-Tronco Neurais / Manganês Tipo de estudo: Estudo diagnóstico Limite: Animais Idioma: Inglês Revista: Chinese Medical Journal Ano de publicação: 2011 Tipo de documento: Artigo