Labeling of human insulin-like growth factor-I eukaryotic expression vector with green fluorescent protein / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
;
(6): 132-137, 2005.
Artigo
em Inglês
| WPRIM
| ID: wpr-338628
ABSTRACT
<p><b>OBJECTIVE</b>To label human insulin-like growth factor-I (hIGF-I) eukaryotic expression vector with green fluorescent protein (GFP) for the repair of articular cartilage defects.</p><p><b>METHODS</b>GFP cDNA was inserted into pcDNA(3.1)-hIGF-1 to construct the co-expression vector with two multiple cloning sites mammalian expression vector under two cytomegalovirus promoters/enhancers respectively. Recombinant pcGI was transfected into NIH 3T3 cells with the help of lipofectamine.</p><p><b>RESULTS</b>Enzyme digestion and agarose gel electrophoresis analysis revealed that pcGI vector contained correct GFP and hIGF-I cDNA. Expression of hIGF-1 and GFP was confirmed in transfected NIH 3T3 cells by immunocytochemical analysis and fluorescence microscopy.</p><p><b>CONCLUSIONS</b>hIGF-I eukaryotic expression vector has been successfully labeled with GFP.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fisiologia
/
Terapêutica
/
Ferimentos e Lesões
/
Fator de Crescimento Insulin-Like I
/
Imuno-Histoquímica
/
Transfecção
/
Terapia Genética
/
Cartilagem Articular
/
Células Cultivadas
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Chinese Journal of Traumatology
Ano de publicação:
2005
Tipo de documento:
Artigo
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